rna-seq protocols Search Results


cats small rna-seq kit (kit c05010044, protocol v.2|09.17  (DIAGENODE DIAGNOSTICS)
90
DIAGENODE DIAGNOSTICS cats small rna-seq kit (kit c05010044, protocol v.2|09.17
Study design and workflow metrics. A) Schematic of study design is shown. MUR, MUR-D, and HBR were processed using 9 different smRNA profiling methods at 4 sites each. The general methodologies included sequential ligation (Illumina, TriLink, Qiagen, NEB, PerkinElmer, Lexogen), template switching (Takara, Diagenode), circularization (Somagenics), and NanoString probe-based hybridization. B) Total start-to-finish preparation time for each kit as reported by sites. C) Mean “ease of use” reported by each site (scale: 1 = uncomfortable, 5 = comfortable). D) Library preparation success rates. Light color blocks are successfully produced libraries. Dark colors are failed libraries. CLO and CLO-S, Takara Bio (Clontech) SMARTer smRNA-Seq Kit; DIA, Diagenode <t>CATS</t> <t>Small</t> <t>RNA-Seq</t> Kit; ILL and ILMN, Illumina TruSeq <t>Small</t> <t>RNA</t> Library Prep Kit; LEX, Lexogen Small RNA-Seq Library Prep Kit; NANO, NanoString nCounter miRNA Expression Assay; NEB, New England Biolabs NEBNext Small RNA Library Prep Set; PEB, PerkinElmer NextFlex Small RNA-Seq Kit v.3; QIA, Qiagen QIAseq miRNA Library Kit; SOM, Somagenics RealSeq-AC miRNA Library Kit; TRI, Trilink CleanTag Small RNA Library Prep Kit.
Cats Small Rna Seq Kit (Kit C05010044, Protocol V.2|09.17, supplied by DIAGENODE DIAGNOSTICS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rna-seq+protocols/pmc06953595-255-1-9?v=DIAGENODE+DIAGNOSTICS
Average 90 stars, based on 1 article reviews
cats small rna-seq kit (kit c05010044, protocol v.2|09.17 - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
3’ rna sequencing  (Lexogen GmbH)
90
Lexogen GmbH 3’ rna sequencing
Study design and workflow metrics. A) Schematic of study design is shown. MUR, MUR-D, and HBR were processed using 9 different smRNA profiling methods at 4 sites each. The general methodologies included sequential ligation (Illumina, TriLink, Qiagen, NEB, PerkinElmer, Lexogen), template switching (Takara, Diagenode), circularization (Somagenics), and NanoString probe-based hybridization. B) Total start-to-finish preparation time for each kit as reported by sites. C) Mean “ease of use” reported by each site (scale: 1 = uncomfortable, 5 = comfortable). D) Library preparation success rates. Light color blocks are successfully produced libraries. Dark colors are failed libraries. CLO and CLO-S, Takara Bio (Clontech) SMARTer smRNA-Seq Kit; DIA, Diagenode <t>CATS</t> <t>Small</t> <t>RNA-Seq</t> Kit; ILL and ILMN, Illumina TruSeq <t>Small</t> <t>RNA</t> Library Prep Kit; LEX, Lexogen Small RNA-Seq Library Prep Kit; NANO, NanoString nCounter miRNA Expression Assay; NEB, New England Biolabs NEBNext Small RNA Library Prep Set; PEB, PerkinElmer NextFlex Small RNA-Seq Kit v.3; QIA, Qiagen QIAseq miRNA Library Kit; SOM, Somagenics RealSeq-AC miRNA Library Kit; TRI, Trilink CleanTag Small RNA Library Prep Kit.
3’ Rna Sequencing, supplied by Lexogen GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rna-seq+protocols/10__1164_slash_rccm__201904___0832le-31-1-9?v=Lexogen+GmbH
Average 90 stars, based on 1 article reviews
3’ rna sequencing - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
rnaseq data processing protocol  (GeneLAB GmbH)
90
GeneLAB GmbH rnaseq data processing protocol
Study design and workflow metrics. A) Schematic of study design is shown. MUR, MUR-D, and HBR were processed using 9 different smRNA profiling methods at 4 sites each. The general methodologies included sequential ligation (Illumina, TriLink, Qiagen, NEB, PerkinElmer, Lexogen), template switching (Takara, Diagenode), circularization (Somagenics), and NanoString probe-based hybridization. B) Total start-to-finish preparation time for each kit as reported by sites. C) Mean “ease of use” reported by each site (scale: 1 = uncomfortable, 5 = comfortable). D) Library preparation success rates. Light color blocks are successfully produced libraries. Dark colors are failed libraries. CLO and CLO-S, Takara Bio (Clontech) SMARTer smRNA-Seq Kit; DIA, Diagenode <t>CATS</t> <t>Small</t> <t>RNA-Seq</t> Kit; ILL and ILMN, Illumina TruSeq <t>Small</t> <t>RNA</t> Library Prep Kit; LEX, Lexogen Small RNA-Seq Library Prep Kit; NANO, NanoString nCounter miRNA Expression Assay; NEB, New England Biolabs NEBNext Small RNA Library Prep Set; PEB, PerkinElmer NextFlex Small RNA-Seq Kit v.3; QIA, Qiagen QIAseq miRNA Library Kit; SOM, Somagenics RealSeq-AC miRNA Library Kit; TRI, Trilink CleanTag Small RNA Library Prep Kit.
Rnaseq Data Processing Protocol, supplied by GeneLAB GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rna-seq+protocols/pmc09576569-234-0-7?v=GeneLAB+GmbH
Average 90 stars, based on 1 article reviews
rnaseq data processing protocol - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier

Image Search Results


Study design and workflow metrics. A) Schematic of study design is shown. MUR, MUR-D, and HBR were processed using 9 different smRNA profiling methods at 4 sites each. The general methodologies included sequential ligation (Illumina, TriLink, Qiagen, NEB, PerkinElmer, Lexogen), template switching (Takara, Diagenode), circularization (Somagenics), and NanoString probe-based hybridization. B) Total start-to-finish preparation time for each kit as reported by sites. C) Mean “ease of use” reported by each site (scale: 1 = uncomfortable, 5 = comfortable). D) Library preparation success rates. Light color blocks are successfully produced libraries. Dark colors are failed libraries. CLO and CLO-S, Takara Bio (Clontech) SMARTer smRNA-Seq Kit; DIA, Diagenode CATS Small RNA-Seq Kit; ILL and ILMN, Illumina TruSeq Small RNA Library Prep Kit; LEX, Lexogen Small RNA-Seq Library Prep Kit; NANO, NanoString nCounter miRNA Expression Assay; NEB, New England Biolabs NEBNext Small RNA Library Prep Set; PEB, PerkinElmer NextFlex Small RNA-Seq Kit v.3; QIA, Qiagen QIAseq miRNA Library Kit; SOM, Somagenics RealSeq-AC miRNA Library Kit; TRI, Trilink CleanTag Small RNA Library Prep Kit.

Journal: Journal of Biomolecular Techniques : JBT

Article Title: Multisite Evaluation of Next-Generation Methods for Small RNA Quantification

doi: 10.7171/jbt.20-3102-001

Figure Lengend Snippet: Study design and workflow metrics. A) Schematic of study design is shown. MUR, MUR-D, and HBR were processed using 9 different smRNA profiling methods at 4 sites each. The general methodologies included sequential ligation (Illumina, TriLink, Qiagen, NEB, PerkinElmer, Lexogen), template switching (Takara, Diagenode), circularization (Somagenics), and NanoString probe-based hybridization. B) Total start-to-finish preparation time for each kit as reported by sites. C) Mean “ease of use” reported by each site (scale: 1 = uncomfortable, 5 = comfortable). D) Library preparation success rates. Light color blocks are successfully produced libraries. Dark colors are failed libraries. CLO and CLO-S, Takara Bio (Clontech) SMARTer smRNA-Seq Kit; DIA, Diagenode CATS Small RNA-Seq Kit; ILL and ILMN, Illumina TruSeq Small RNA Library Prep Kit; LEX, Lexogen Small RNA-Seq Library Prep Kit; NANO, NanoString nCounter miRNA Expression Assay; NEB, New England Biolabs NEBNext Small RNA Library Prep Set; PEB, PerkinElmer NextFlex Small RNA-Seq Kit v.3; QIA, Qiagen QIAseq miRNA Library Kit; SOM, Somagenics RealSeq-AC miRNA Library Kit; TRI, Trilink CleanTag Small RNA Library Prep Kit.

Article Snippet: Diagenode CATS Small RNA-Seq Kit (kit C05010044, protocol v.2|09.17; Diagenode, Liège, Belgium) The amount of input RNA for HBR and MUR-D was 10 ng and 35 pg for MUR.

Techniques: Ligation, Hybridization, Produced, RNA Sequencing Assay, Expressing

miRNA detection and bias. Percentages of Miltenyi Biotec miRXplore miRNA detected above 5 CPM in MUR (A) and in MUR-D (B) are shown. Percentages and amplitude of Miltenyi Biotec miRXplore miRNA detected that deviated from the median are shown in MUR (C) or MUR-D (D). The darkest shade is within 2-fold of the median; the medium shade is 2–10-fold either up or down vs. the expected value; the lightest shade is >10× either increased or decreased. Percentages of reads increased >10× from median in MUR (E) or MUR-D (F). CLO-S and C, Takara Bio (Clontech) SMARTer smRNA-Seq Kit; DIA and D, Diagenode CATS Small RNA-Seq Kit; ILMN and I, Illumina TruSeq Small RNA Library Prep Kit; LEX and L, Lexogen Small RNA-Seq Library Prep Kit; Nano, NanoString nCounter miRNA Expression Assay; NEB and N, New England Biolabs NEBNext Small RNA Library Prep Set; PEB and P, PerkinElmer NextFlex Small RNA-Seq Kit v.3; QIA and Q, Qiagen QIAseq miRNA Library Kit; SOM and S, Somagenics RealSeq-AC miRNA Library Kit; TRI and T, Trilink CleanTag Small RNA Library Prep Kit.

Journal: Journal of Biomolecular Techniques : JBT

Article Title: Multisite Evaluation of Next-Generation Methods for Small RNA Quantification

doi: 10.7171/jbt.20-3102-001

Figure Lengend Snippet: miRNA detection and bias. Percentages of Miltenyi Biotec miRXplore miRNA detected above 5 CPM in MUR (A) and in MUR-D (B) are shown. Percentages and amplitude of Miltenyi Biotec miRXplore miRNA detected that deviated from the median are shown in MUR (C) or MUR-D (D). The darkest shade is within 2-fold of the median; the medium shade is 2–10-fold either up or down vs. the expected value; the lightest shade is >10× either increased or decreased. Percentages of reads increased >10× from median in MUR (E) or MUR-D (F). CLO-S and C, Takara Bio (Clontech) SMARTer smRNA-Seq Kit; DIA and D, Diagenode CATS Small RNA-Seq Kit; ILMN and I, Illumina TruSeq Small RNA Library Prep Kit; LEX and L, Lexogen Small RNA-Seq Library Prep Kit; Nano, NanoString nCounter miRNA Expression Assay; NEB and N, New England Biolabs NEBNext Small RNA Library Prep Set; PEB and P, PerkinElmer NextFlex Small RNA-Seq Kit v.3; QIA and Q, Qiagen QIAseq miRNA Library Kit; SOM and S, Somagenics RealSeq-AC miRNA Library Kit; TRI and T, Trilink CleanTag Small RNA Library Prep Kit.

Article Snippet: Diagenode CATS Small RNA-Seq Kit (kit C05010044, protocol v.2|09.17; Diagenode, Liège, Belgium) The amount of input RNA for HBR and MUR-D was 10 ng and 35 pg for MUR.

Techniques: RNA Sequencing Assay, Expressing

Relative expression of miRNAs. A) Correlation scatter plots of miRNAs detected in MUR and MUR-D for the QIA method, with miRNAs scored in log2 mapped reads per million. B) Correlation scatter plot of miRNAs detected in MUR-D using LEX vs. ILL kits, with miRNAs scored in log2 mapped reads per million. C) Unsupervised hierarchical clustering heatmap of log2 transformed CPM across all methods. C (prep kit), Takara Bio (Clontech) SMARTer smRNA-Seq Kit; C (prep type), circularization; D, Diagenode CATS Small RNA-Seq Kit; ILL and I, Illumina TruSeq Small RNA Library Prep Kit; L (prep type), sequential ligation; LEX and L (prep kit), Lexogen Small RNA-Seq Library Prep Kit; N, New England Biolabs NEBNext Small RNA Library Prep Set; Nano, NanoString nCounter miRNA Expression Assay; NO, No size selection; P, PerkinElmer NextFlex Small RNA-Seq Kit v.3; QIA and Q, Qiagen QIAseq miRNA Library Kit; S, Somagenics RealSeq-AC miRNA Library Kit; SAGE, pippin prep; SPRI, solid phase reversible immobilization; T (prep kit), Trilink CleanTag Small Library Prep Kit; T (prep type), template switching.

Journal: Journal of Biomolecular Techniques : JBT

Article Title: Multisite Evaluation of Next-Generation Methods for Small RNA Quantification

doi: 10.7171/jbt.20-3102-001

Figure Lengend Snippet: Relative expression of miRNAs. A) Correlation scatter plots of miRNAs detected in MUR and MUR-D for the QIA method, with miRNAs scored in log2 mapped reads per million. B) Correlation scatter plot of miRNAs detected in MUR-D using LEX vs. ILL kits, with miRNAs scored in log2 mapped reads per million. C) Unsupervised hierarchical clustering heatmap of log2 transformed CPM across all methods. C (prep kit), Takara Bio (Clontech) SMARTer smRNA-Seq Kit; C (prep type), circularization; D, Diagenode CATS Small RNA-Seq Kit; ILL and I, Illumina TruSeq Small RNA Library Prep Kit; L (prep type), sequential ligation; LEX and L (prep kit), Lexogen Small RNA-Seq Library Prep Kit; N, New England Biolabs NEBNext Small RNA Library Prep Set; Nano, NanoString nCounter miRNA Expression Assay; NO, No size selection; P, PerkinElmer NextFlex Small RNA-Seq Kit v.3; QIA and Q, Qiagen QIAseq miRNA Library Kit; S, Somagenics RealSeq-AC miRNA Library Kit; SAGE, pippin prep; SPRI, solid phase reversible immobilization; T (prep kit), Trilink CleanTag Small Library Prep Kit; T (prep type), template switching.

Article Snippet: Diagenode CATS Small RNA-Seq Kit (kit C05010044, protocol v.2|09.17; Diagenode, Liège, Belgium) The amount of input RNA for HBR and MUR-D was 10 ng and 35 pg for MUR.

Techniques: Expressing, Transformation Assay, RNA Sequencing Assay, Ligation, Selection

Detection of smRNAs in complex samples. Insert sizes in MUR-D (A) and HBR (B) are shown with inserts <18 nt as white, 18–23 nt as the lightest shade, 24–35 nt as the medium shade, and >35 nt as the darkest shade. Data from the different method types are colored (ligation, blue; polyA tailing, orange; circularization, green). The right panels show the smRNA target distribution for MUR-D (C) and HBR (D). Mapped targets are noted in the key. Unmapped targets are shown in black. C, Takara Bio (Clontech) SMARTer smRNA-Seq Kit; D, Diagenode CATS Small RNA-Seq Kit; I, Illumina TruSeq Small RNA Library Prep Kit; L, Lexogen Small RNA-Seq Library Prep Kit; lincRNA, long intervening noncoding RNA; N, New England Biolabs NEBNext Small RNA Library Prep Set; ncRNA, noncoding RNA; P, PerkinElmer NextFlex Small RNA-Seq Kit v.3; Q, Qiagen QIAseq miRNA Library Kit; S, Somagenics RealSeq-AC miRNA Library Kit; snoRNA, small nucleolar RNA; snRNA, small nuclear RNA; T, Trilink CleanTag Small Library Prep Kit.

Journal: Journal of Biomolecular Techniques : JBT

Article Title: Multisite Evaluation of Next-Generation Methods for Small RNA Quantification

doi: 10.7171/jbt.20-3102-001

Figure Lengend Snippet: Detection of smRNAs in complex samples. Insert sizes in MUR-D (A) and HBR (B) are shown with inserts <18 nt as white, 18–23 nt as the lightest shade, 24–35 nt as the medium shade, and >35 nt as the darkest shade. Data from the different method types are colored (ligation, blue; polyA tailing, orange; circularization, green). The right panels show the smRNA target distribution for MUR-D (C) and HBR (D). Mapped targets are noted in the key. Unmapped targets are shown in black. C, Takara Bio (Clontech) SMARTer smRNA-Seq Kit; D, Diagenode CATS Small RNA-Seq Kit; I, Illumina TruSeq Small RNA Library Prep Kit; L, Lexogen Small RNA-Seq Library Prep Kit; lincRNA, long intervening noncoding RNA; N, New England Biolabs NEBNext Small RNA Library Prep Set; ncRNA, noncoding RNA; P, PerkinElmer NextFlex Small RNA-Seq Kit v.3; Q, Qiagen QIAseq miRNA Library Kit; S, Somagenics RealSeq-AC miRNA Library Kit; snoRNA, small nucleolar RNA; snRNA, small nuclear RNA; T, Trilink CleanTag Small Library Prep Kit.

Article Snippet: Diagenode CATS Small RNA-Seq Kit (kit C05010044, protocol v.2|09.17; Diagenode, Liège, Belgium) The amount of input RNA for HBR and MUR-D was 10 ng and 35 pg for MUR.

Techniques: Ligation, RNA Sequencing Assay